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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,180)
Mouse Secondary Antibodies (14,490)
Rabbit Secondary Antibodies (6,301)
Donkey Secondary Antibodies (4,319)
Rat Secondary Antibodies (1,234)
Sheep Secondary Antibodies (701)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Pig Secondary Antibodies (76)
Horse Secondary Antibodies (74)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (57)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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5,161 – 5,175 of 71,978 results
5,161

Goat anti-Canine IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Canine
Host Species Goat
Conjugate FITC
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin)
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500
Classification Polyclonal
Immunogen Dog IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with dog IgG and with light chains common to other dog immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
5,162

Goat anti-Human Kappa Light Chain Secondary Antibody, HRP, Novus Biologicals™
SDP

Goat Polyclonal Antibody has been used in 6 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Human
Host Species Goat
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Concentration 1.0 mg/mL
Antigen Kappa Light Chain
Gene Symbols IGKC
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:20000, ELISA 1:10000 - 1:100000, Immunohistochemistry 1:200 - 1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2000, Immunohistochemistry-Paraffin
Gene Alias HCAK1, IGKCD, immunoglobulin kappa constant, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809
Gene ID (Entrez) 3514
Classification Polyclonal
Immunogen Human Kappa Light Chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with kappa light chains common to all human immunoglobulins. No was detected against lambda light chain or non-immunoglobulin serum proteins.
5,163

Goat anti-Mouse Lambda Light Chain Secondary Antibody, Novus Biologicals™
SDP

Goat Polyclonal Antibody has been used in 3 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Mouse
Host Species Goat
Conjugate Unconjugated
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Concentration 1.0 mg/mL
Antigen Lambda Light Chain
Gene Symbols IGLC1
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1000 - 1:30000, ELISA 1:1000 - 1:30000, Immunohistochemistry 1:200- 1:2000, Immunocytochemistry/Immunofluorescence 1:200- 1:2000, Immunohistochemistry-Paraffin
Gene Alias IGLC, immunoglobulin lambda constant group
Gene ID (Entrez) 3536
Classification Polyclonal
Immunogen Mouse Lambda Light Chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with lambda light chains common to all mouse immunoglobulins. No was detected against kapp light chain or non-immunoglobulin serum proteins.
5,164

Goat anti-Human IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody has been used in 2 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Human
Host Species Goat
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:5000 - 1:50000, ELISA 1:10000- 1:100000, Immunohistochemistry 1:200 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:5000
Classification Polyclonal
Immunogen Human IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using rhesus and cynomolgus monkey immunosorbents to remove cross reactive Antibodies. Less than 0.1% cross reactivity to monkey IgG was detected.
5,165

Goat anti-Guinea Pig IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
SDP

Goat Polyclonal Antibody has been used in 2 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Guinea Pig
Host Species Goat
Conjugate HRP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:5000-1:50000, ELISA 1:10000-1:100000, Immunohistochemistry 1:200 - 1:5000, Immunocytochemistry/Immunofluorescence 1:200 - 1:5000, Immunohistochemistry-Paraffin 1:200 - 1:5000
Classification Polyclonal
Immunogen Guinea Pig IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this antibody reacts specifically with guinea pig IgG and with light chains common to other guinea pig immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. This antibody may cross react with IgG from other species.
5,166

Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Goat
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:4000 - 1:20000, ELISA 1:20000 - 1:80000, Immunohistochemistry 1:1000 - 1:8000
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Human Serum Proteins.
5,167

Goat anti-Human IgA/IgG/IgM (H+L) Secondary Antibody, Texas Red, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Human
Host Species Goat
Conjugate Texas Red
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Concentration LYOPH
Antigen IgA/IgG/IgM (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Human IgG, IgA and IgM whole molecules
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.
5,168

Goat anti-Mouse IgA/IgG/IgM (H+L) Secondary Antibody, Rhodamine (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Goat
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Concentration LYOPH
Antigen IgA/IgG/IgM (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Mouse IgG IgA and IgM whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Bovine, Horse, and Human Serum Proteins. No reaction was observed against bovine, horse or human serum proteins. This reagent is suitable for the detection of all mouse immunoglobulin isotypes and chain combinations.
5,169

Donkey anti-Sheep IgG (H+L) Secondary Antibody, DyLight 405 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate DyLight 405
Applications Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins. This antibody will react with heavy chains of Sheep IgG and with light chains of most Sheep immunoglobulins.
5,170

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 488 (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 2 publications

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Goat
Host Species Donkey
Conjugate DyLight 488
Applications Dot Blot,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:10000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:5000, Immunohistochemistry-Frozen, Dot Blot, Fluorophore-linked immunosorbent assay 1:20000
Classification Polyclonal
Immunogen Goat IgG whole molecule
Reconstitution Reconstitute with 100 ul deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
5,171

Goat anti-Bovine IgG F(ab')2 Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Bovine
Host Species Goat
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Concentration LYOPH
Antigen IgG F(ab')2
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Gene Alias immunoglobulin heavy constant gamma 1 (G1m marker)
Classification Polyclonal
Immunogen Bovine IgG F(ab')2 fragment
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity No reaction was observed against Bovine IgG F(c).
5,172

Goat anti-Rabbit IgG Fc Secondary Antibody, Biotin (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Target Species Rabbit
Host Species Goat
Conjugate Biotin
Applications ELISA,Immunohistochemistry,Western Blot
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:2000 - 1:10000, ELISA 1:200000, Immunohistochemistry 1:1000 - 1:5000
Classification Polyclonal
Immunogen Goat anti-Rabbit IgG Fc fragment Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with rabbit IgG F(c) fragment in goat.
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Human Serum Proteins. No reaction was observed against Rabbit IgG F(ab) or Human Serum Proteins.
5,173

Goat anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Mouse
Host Species Goat
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Mouse IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins. This antibody has been pre-adsorbed against bovine, goat, human, rabbit, and rat IgG.
5,174

Goat anti-Rabbit IgG Fc Secondary Antibody, DyLight 550, Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Goat
Conjugate DyLight 550
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Concentration LYOPH
Antigen IgG Fc
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG, Fc fragment
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG
5,175

Goat anti-Human IgM Heavy Chain Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
SDP

Goat Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Human
Host Species Goat
Conjugate HRP
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgM
Concentration LYOPH
Antigen IgM Heavy Chain
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Gene Alias Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain
Gene ID (Entrez) 3507
Classification Polyclonal
Immunogen Purified Human IgM
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy mu chains on human IgM. This antibody has been pre-adsorbed against human IgA + IgG.